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G005724

HPLC Analysis of Proteins on BIOshell A160 Peptide C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase: [A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile
gradient: held at 0% B for 0.5 min; 0 to 100% B in 7 min
flow rate: 0.3 mL/min
pressure: 4743 psi (327 bar)
column temp.: 35 °C
detector: UV, 220 nm
injection: 1 μL
sample: 0.2 - 0.6 g/L in mobile phase A

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Analyte

Description

    Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
    Adrenomedullin rat, ≥95% (HPLC)
    Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein
    Superoxide Dismutase from bovine erythrocytes, BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell culture
    Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
    Carbonic Anhydrase from bovine erythrocytes, lyophilized powder, ≥2,000 W-A units/mg protein
    Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
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