ZRB1970

Anti-IL-6 Antibody, clone 1G18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Interleukin-6, B-cell hybridoma growth factor, Interleukin HP-1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1G18, recombinant monoclonal
• description: 1G18 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 24.38 kDa; observed mol wt ~18 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_001300983
• UniProt accession no.: P08505
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... IL6(16193)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Interleukin-6 (IL-6). It targets an epitope within 23 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of mouse IL-6.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant mouse IL-6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant mouse IL-6.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound murine IL-6 peptide with a KD of 1.6 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL-6 in mouse spleen and human tonsil tissue sections.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected IL-6 in one million RAW264.7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Interleukin-6 (UniProt: P08505; also known as IL-6, B-cell hybridoma growth factor, Interleukin HP-1) is encoded by the IL6 (also known as IL-6) gene (Gene ID: 16193) in murine species. IL-6 is an immunoregulatory secreted cytokine that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to produce the mature form (aa 25-211). It is expressed by dendritic cells, macrophages, and activated follicular B cells. It is also abundantly expressed in the central nervous system, particularly in the hypothalamic region. It is a potent inducer of the acute phase response and rapid IL-6 production contributes to host defense during infection and tissue injury. In the innate immune response, it is synthesized by myeloid cells upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection. In the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It is reported to plays a major role in the differentiation of CD4+ T cell subsets and is an essential factor for the development of T follicular helper (Tfh) cells. IL-6 also acts as an essential factor in bone homeostasis and acts directly or indirectly on blood vessels by induction of VEGF that results in increased angiogenesis activity and vascular permeability. Its actions are mediated by binding to IL-6 receptor (IL-6R), and the ligand-receptor complex associates to the signaling subunit IL6ST/gp130. The interaction with the membrane-bound IL6R and IL6ST is reported to stimulate the classic signaling, whereas the binding of IL-6 and soluble IL6R to IL6ST stimulates trans-signaling where synergistically with IL-1β and TNF it can induce the production of VEGF and stimulate angiogenesis. (Ref.: Heink, S., et al. (2017). Nat. Immunol. 18(1); 74-85; Nakahara, H., et al. (2003). Arthritis Rheum. 48(6); 1521-1529).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable