ZRB1156
Anti-MeCP2 Antibody, clone 1L20 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
About This Item
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1L20, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 52.44 kDa
observed mol wt ~75 kDa
purified by
using Protein A
species reactivity
monkey, mouse, human
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
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technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in Cos-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in Cos-7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MeCP2 in MCF-7 and HeLa cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MeCP2 in A431, HeLa, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MeCP2 in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound MeCP2 with a KD of 9.6 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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