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Key Documents

SAB3500471

Sigma-Aldrich

Monoclonal Anti-EndoG antibody produced in mouse

clone 7F2G10, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Endonuclease G

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About This Item

MDL number:
MFCD04040310
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7F2G10, monoclonal

form

buffered aqueous solution

species reactivity

rat, human

technique(s)

indirect ELISA: suitable
western blot: suitable

NCBI accession no.

Q14249

UniProt accession no.

Q14249

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ENDOG(2021)

General description

ENDOG (Endonuclease G) is a 28kDa mitochondria-localized protein mapped to human chromosome 9q34.1. However, in presence of certain conditions it can translocate to the nucleus through the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway.

Immunogen

Mouse monoclonal EndoG antibody was raised against a recombinant protein corresponding to amino acids 51 – 140 of human EndoG.

Application

Monoclonal Anti-EndoG antibody produced in mouse is suitable for indirect ELISA and western blot.

Biochem/physiol Actions

ENDOG (Endonuclease G) is a mitochondrial apoptotic DNase involved in the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. During mitochondrial release and nuclear translocation of EndoG, BNIP3 interacts with the voltage-dependent anion channel (VDAC) to facilitate the process in the nucleus, it cleaves chromatin apoptotic DNA without the need of caspase activity. ENDOG causes DNA fragmentation during and after apoptosis. It is linked to cardiac hypertrophy and Parkinson′s disease.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in PBS with 0.02% sodium azide.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Dae Song Jang et al.
DNA and cell biology, 34(2), 92-100 (2014-11-18)
Apoptotic endonuclease G (EndoG) is responsible for DNA fragmentation both during and after cell death. Previous studies demonstrated that genetic inactivation of EndoG is cytoprotective against various pro-apoptotic stimuli; however, specific inhibitors for EndoG are not available. In this study
Xiaosha Zhang et al.
PloS one, 9(12), e113642-e113642 (2014-12-02)
BNIP3 is a proapoptotic protein that induces cell death through a mitochondria-mediated pathway. We reported previously that mitochondrial localization of BNIP3 and translocation of EndoG from mitochondria to the nucleus are critical steps of the BNIP3 pathway. It is not
V Tiranti et al.
Genomics, 25(2), 559-564 (1995-01-20)
By using a PCR-based screening of a somatic cell hybrid panel and FISH, we have assigned the loci of mitochondrial single-stranded DNA-binding protein (SSBP), mitochondrial transcription factor A (TCF6), and mitochondrial endonuclease G (ENDOG) genes to human chromosomes 7q34, 10q21

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