R5780

Anti-Rab4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-HRES-1/RAB4, Anti-RAB4A, Anti-RAS-associated protein RAB4
• MDL number: MFCD01634072
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~24 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.1% and 0.4% saponin, respectively.; western blot: 2-5 μg/mL using whole extract of human HEK-293T cells
• UniProt accession no.: P20338
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB4A(5867) ; mouse ... Rab4a(19341) ; rat ... Rab4a(25532)

Description

General description

Rab4 is a GTPase , that is located in early and recycling endosomes. It belongs to Rab family and is located on human chromosome 1q42. In rat testis, Rab4 is found in sertoli and germ cells.

There are two highly homologous RAB4 isoforms, RAB4A and RAB4B, encoded by two separate genes (RAB4A and RAB4B). RAB4A and RAB4B are localized in the same cellular compartments and are believed to have similar or identical functions in recycling.

Application

The antibody can be used in several immunochemical techniques including immunoblotting (24 kDa) and immunofluorescence.

Biochem/physiol Actions

Rab4 regulates endocytic recycling in a cell cycle dependent fashion. Rab4 is associated with early endosomes and recycling endosomes and regulates the recycling of membranes and proteins from these compartments back to the plasma membrane. Rab4 interacts with several Rab4 effector proteins like Rabip4, that regulates integrin trafficking and is involved in the migration of NIH 3T3 fibroblasts.

Rab4a controls integrin trafficking throughout cell adhesion. It helps in the quick reprocess of receptors from early endosomes to the cell surface. It also controls the secretion of procathepsin L in human melanoma cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves