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Sigma-Aldrich

TruPAGE Precast Gels

4-12%, 10 x 8cm, 12-well

Synonym(s):

Precast Gels for SDS-PAGE, Precast Polyacrylamide Gels

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About This Item

UNSPSC Code:
41105300
NACRES:
NB.22

description

for use with Bio-Rad Mini-PROTEAN™ Tetra Cell, Mini-PROTEAN Dodeca™, Mini-PROTEAN II and 3 cells

shelf life

24 mo. at 2‑8 °C (from the date of manufacture)

packaging

box of 10 ea

concentration

4-12% polyacrylamide

cassette W × L

10 cm × 8 cm

gel thickness

1 mm

well size × volume

12 wells × 35 μL

compatibility

for use with Hoefer Mighty Small II and Tall Mighty Small (SE 260 and SE280)

storage temp.

2-8°C

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Features and Benefits

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The gels come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.
TruPAGE Precast Gels Migration Chart

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yunjiao Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(31), 7547-7559 (2017-07-01)
Calcium-dependent nuclear export of histone deacetylase 1 (HDAC1) was shown previously to precede axonal damage in culture, but the in vivo relevance of these findings and the potential posttranslational modifications of HDAC1 remained elusive. Using acute hippocampal slices from mice

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