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P7437

Sigma-Aldrich

Monoclonal Anti-CD19−PE antibody produced in mouse

clone SJ25-C1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-CD19

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About This Item

MDL number:
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SJ25-C1, monoclonal

form

buffered aqueous solution

technique(s)

flow cytometry: 10 μL using 1 × 106 cells

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD19(930)

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Specificity

Recognizes the human CD19 (90 kDa) integral membrane glycoprotein.
3rd Workshop: code no. 073

Immunogen

NALM-1 human cell line.

Target description

CD19 is a member of the immunoglobulin superfamily and has two Ig-like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of κ or λ light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Nakamura et al.
FEBS letters, 463(1-2), 125-128 (1999-12-22)
We have investigated the regulation mechanism of the surface sialyl-Le(X) (sLe(X)) expression level in tonsillar B cells during activation. sLe(X) antigen became strongly positive after activation, while resting B cells were weakly positive. sLe(X) structures were mainly located on O-linked

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