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P5475

Sigma-Aldrich

C-CMV-24 Sequencing Primer

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About This Item

MDL number:
UNSPSC Code:
12352200

grade

for molecular biology

shipped in

dry ice

storage temp.

−20°C

General description

The C-CMV-24 sequencing primer is a chemically synthesized, 24 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the N-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 1116-1139 of negative, non-coding DNA strand of the pFLAG-CMV-1 vector and nucleotides 1073-1096 of negative, non-coding DNA strand of the pFLAG-CMV-2 vector.

Application

The N-CMV-30 sequencing primer is a chemically synthesized, 30 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the C-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 818-847 of positive, coding DNA strand of the pFLAG-CMV vectors.

Components

The primer is supplied at an initial concentration of 5 uM in 1xTE buffer. The nucleotide sequence is as follows: 5′-TAT-TAG-GAC-AAG-GCT-GGT-GGG-CAC-3′

Other Notes

(5′-TAT TAG GAC AAG GCT GGT GGG CAC-3′)

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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