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MAK055

Sigma-Aldrich

AST Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Aspartate Aminotransferase Activity Assay Kit, Glutamate-Oxaloacetate Transaminase Activity Assay Kit, SGOT Activity Assay Kit, Serum Glutamate-Oxaloacetate Transaminase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

General description

The new AST Assay Kit, MAK467, is now available! Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.

Application

AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.[1][2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum

Principle

The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 μmole of glutamate per minute at pH 8.0 at 37 °C.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8A06K07530
8M08K07530
8D13K07530
8G15K07530
8H22K07530

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is it possible to use the MAK055 for an experiment that involves evaluating samples with and without B6 added? Additionally, will hemoglobin interfere if a blood hemolysate sample is used when assayed with this kit?

    1 answer

    1. The specific experiment in question has not been performed in the lab, and there are no references for the Aspartate Aminotransferase Activity Assay Kit where a similar experiment has been reported. Considering the nature of the assay and the fact that B6 is a co-enzyme of the ASP, necessary for its activity, in principle, the kit will be suitable to evaluate samples treated with B6. It will be necessary to evaluate any possible interferences and optimize user-specific conditions.
      It is possible that hemoglobin will interfere with the assay if hemolyzed blood is tested. Blood is generally not recommended as a sample, unless specifically mentioned in the assay, due to the possible interference as mentioned. Furthermore, the kit has not been tested with blood samples.

      Helpful?

  4. Does the AST Activity Assay Kit (MAK055), which includes a positive control (MAK055F), have a specific species origin? For instance, is it Human AST?

    1 answer

    1. The positive control in MAK055 is derived from porcine heart.

      Helpful?

  5. Do you anticipate phenol red in culture medium having an impact on the colorimetric read out at 450 nm? (I'm thinking there may be some spectral overlap)

    1 answer

    1. Ideally, culture media should be phenol red-free.

      Helpful?

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