product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
M1255
Calcein Blue
Powder
Synonym(s):
4-Methylumbelliferone-8-methyliminodiacetic acid
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All Photos(3)
About This Item
Empirical Formula (Hill Notation):
C15H15NO7
CAS Number:
Molecular Weight:
321.28
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Product Name
Calcein Blue, fluorescent dye
Quality Level
form
powder
technique(s)
titration: suitable
color
white to light yellow
solubility
1 M NaOH: 50 mg/mL
fluorescence
λex 322 nm; λem 445 nm in methanol
suitability
suitable for indicator for EDTA titration
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChI key
RJPSHDMGSVVHFA-UHFFFAOYSA-N
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General description
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Application
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?
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