L9150

N-Lauroylsarcosine sodium salt

detergent for use in cell lysis

• Synonym(s): Sodium lauroyl sarcosinate, N-Dodecanoyl-N-methylglycine sodium salt, Sarkosyl NL
• Linear Formula: CH₃(CH₂)₁₀CON(CH₃)CH₂COONa
• CAS Number: 137-16-6
• Molecular Weight: 293.38
• Beilstein: 5322974
• EC Number: 205-281-5
• MDL number: MFCD00042728
• UNSPSC Code: 12161902
• PubChem Substance ID: 57654340
• NACRES: NA.52

Properties

• grade: for molecular biology
• description: anionic
• product line: BioReagent
• Assay: ≥94%
• form: powder
• mol wt: micellar avg mol wt 600
• aggregation number: 2
• technique(s): protein purification: suitable; protein quantification: suitable
• CMC: 14.6 mM (20-25°C)
• solubility: H₂O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• SMILES string: [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O
• InChI: 1S/C15H29NO3.Na/c1-3-4-5-6-7-8-9-10-11-12-14(17)16(2)13-15(18)19;/h3-13H2,1-2H3,(H,18,19);/q;+1/p-1
• InChI key: KSAVQLQVUXSOCR-UHFFFAOYSA-M

Description

General description

Sodium lauroyl sarcosinate, the sodium salt of an acyl derivative of sarcosine—a natural amino acid occurring in muscles and body tissues—stands out as a valuable component in molecular biology, biochemical, and cell biology research. Derived from a natural fatty acid and the amino acid derivative sarcosine, N-Lauroylsarcosine is a biodegradable anionic surfactant usually synthesized through acylation and neutralization reactions involving lauric acid and sodium sarcosine.

This amino acid-type anionic surfactant, also recognized as a fatty acid amide and detergent, exhibits protein denaturant potency and has been integrated into lysing solutions, such as the cornet assay. Its application extends to lysing protoplasts for chromosomal DNA isolation from Streptococcus mutans. Notably, Lauroylsarcosine Sodium Salt, characterized by its ionic detergent properties, is commonly employed for purifying membrane proteins. Additionally, Sodium Lauroyl Sarcosine also plays a crucial role in concentrated salt solutions, facilitating cell lysis in RNA purification protocols and membrane solubilization. Whether it′s breaking down cell structures or facilitating membrane protein purification, this mild surfactant proves valuable in various applications within the realms of biology and biochemistry.

Application

Suitable for

• solubilization and separation of membrane proteins
• lysis of cells during the isolation of RNA
• inhibition of hexokinase

Features and Benefits

• Suitable for Molecular Biology, Biochemical and Cell Biology research
• Can be used for Protein Purification and Quantification
• High purity product for research applications

Other Notes

Anionic surfactant.

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Safety Information

• Pictograms: GHS06,GHS05
• Signal Word: Danger
• Hazard Statements: H315,H318,H330
• Precautionary Statements: P260 - P264 - P280 - P302 + P352 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 1
• Flash Point(F): 512.6 °F - closed cup
• Flash Point(C): 267 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves