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I2511

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (HPLC), buffered aqueous solution

Synonym(s):

Human IgG

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10 MG
R 1 604,26

R 1 604,26

List PriceR 1 637,00

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10 MG
R 1 604,26

About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

R 1 604,26

List PriceR 1 637,00

Please contact Customer Service for Availability

Request a Bulk Order

conjugate

unconjugated

Quality Level

grade

reagent grade

Assay

≥95% (HPLC)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[1]
Human IgG is purified from normal human serum by precipitation and gel filtration techniques.

Application

Purified human IgG has been used :-
  • as blocking antibody for flow cytometry[2]
  • as negative control in chromatin immunoprecipitation assays[3]
  • to precipitate IgG in human larynx epidermoid carcinoma cells by immunoprecipitation[4]
  • as a standard in enzyme-linked immunosorbent assay[5]

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases.[6] IgG1 regulates complement fixation in mice.[5]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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In-Kyu Yoon et al.
The American journal of tropical medicine and hygiene, 72(6), 714-718 (2005-06-21)
Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 1(42) (MSP-1(42)) exhibit antibodies to MSP-1(42) that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-1(42) antibody
Kah Teong Soh et al.
Current protocols in cytometry, 75, 7-7 (2016-01-09)
Nucleic acid content can be quantified by flow cytometry through the use of intercalating compounds; however, measuring the presence of specific sequences has hitherto been difficult to achieve by this methodology. The primary obstacle to detecting discrete nucleic acid sequences
Kentaro Matsuda et al.
The Journal of allergy and clinical immunology, 116(6), 1357-1363 (2005-12-13)
Mouse monoclonal IgE antibodies can promote the survival of mouse bone marrow-derived cultured mast cells and induce the cells to secrete mediators in the absence of known specific antigen. To determine whether human IgE, in the absence of known specific
D R McArthur et al.
Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland, 11(7), 775-782 (2008-08-12)
Trials investigating colorectal cancer (CRC) chemoprophylaxis with cyclooxygenase-2 (COX-2) inhibitors have been discontinued because of adverse cardiovascular effects. Nevertheless, identification of patients where beneficial, chemo-prophylactic effects of COX-2 inhibitors outweigh side-effects may be possible; this study aimed to investigate whether
Charles C Chu et al.
Blood, 112(13), 5122-5129 (2008-09-25)
Leukemic B lymphocytes of a large group of unrelated chronic lymphocytic leukemia (CLL) patients express an unmutated heavy chain immunoglobulin variable (V) region encoded by IGHV1-69, IGHD3-16, and IGHJ3 with nearly identical heavy and light chain complementarity-determining region 3 sequences.

Questions

1–2 of 2 Questions  
  1. I would like to have the full amino acid sequence of the IgG from product I2511. I cannot find this information in any of the data sheets available online

    1 answer
    1. This IgG product exhibits variable domains and has not been sequenced. The material is isolated and purified by precipitation and affinity chromatography. Sequence information on human immunoglobulins is broadly available in published literature. As an example, please see the link below to review the NIH database which includes sequence information:
      https://www.ncbi.nlm.nih.gov/protein/AAA02914.1

      Helpful?

  2. What is the shelf life of item A2661 after reconstitution?

    1 answer
    1. The stock solution can be stored at -20°C for up to 3 months and can be aliquoted for sampling convenience.

      Helpful?

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