HPA040902
Anti-MYO5B antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-Kiaa1119, Anti-Myosin vb
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All Photos(6)
About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
orthogonal RNAseq
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry: 1:200- 1:500
immunogen sequence
NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYO5B(4645)
General description
Myosin VB (MYO5B) gene spanning 372,296 bases on genomic DNA with 42 exons is mapped to human chromosome 18q21.1. The gene codes for ubiquitously expressed myosin Vb protein. MYO5B contains N-terminal motor domain and C-terminal cargo-binding domain.
Immunogen
myosin VB recombinant protein epitope signature tag (PrEST)
Application
Anti-MYO5B antibody produced in rabbit has been used in immunostaining and western blot analysis.
Biochem/physiol Actions
Myosin VB (MYO5B) acts as a motor for actin dependent organelle trafficking. Mutation or loss of MYO5B gene is associated with the development of an autosomal recessive syndrome, microvillus inclusion disease (MVID). Decreased expression of the protein has been observed in patients of gastric cancer. Therefore, MYO5B might act as a potential biomarker for gastric cancer. MYO5B interacts with Ras-related proteins 8a and 11a (Rab8a–Rab11a) component and stimulates stretch-induced exocytosis in bladder umbrella cells.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Linkage
Corresponding Antigen APREST81706
Physical form
Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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An overview and online
registry of microvillus
inclusion disease patients
and their MYO5B mutations
registry of microvillus
inclusion disease patients
and their MYO5B mutations
van der Velde KJ
Human Mutation, 34(12), 1597-1605 (2013)
Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum.
Weis VG
Cellular and molecular gastroenterology and hepatology, 2(2), 131-157 (2016)
Inactivation of MYO5B promotes invasion and motility in gastric cancer cells.
Dong W
Digestive Diseases and Sciences, 57(5), 1247-1252 (2012)
A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.
Khandelwal P
Molecular Biology of the Cell, 24(7), 1007-1019 (2013)
Victoria G Weis et al.
Cellular and molecular gastroenterology and hepatology, 2(2), 131-157 (2016-03-29)
Inactivating mutations in MYO5B cause severe neonatal diarrhea in Microvillus Inclusion Disease. Loss of active MYO5B causes the formation of pathognomonic inclusions and aberrations in brush border enzymes. We developed three mouse models of germline, constitutively intestinal targeted and inducible
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