H6024
Hoechst Stain solution
Synonym(s):
Bisbenzimide H 33258
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form
solution
application(s)
histology
storage temp.
2-8°C
SMILES string
O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6
InChI
1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2
InChI key
OWRSPPSBNWJJAR-UHFFFAOYSA-N
General description
Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells.[1] It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.[2]
Application
A fluorescent DNA stain qualified for use in Mycoplama staining.
Components
Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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