H2537

Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD04040365
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~105 kDa
• species reactivity: mouse, human, rat
• technique(s): immunoprecipitation (IP): 1.5-1.8 μg using RIPA extract of 293T cells expressing recombinant human HDAC7; microarray: suitable; western blot: 1:2,000 using whole extracts of mouse NIH-3T3 cells; western blot: 1:500 using whole extracts of rat PC12 cells
• UniProt accession no.: Q8WUI4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HDAC7(51564) ; mouse ... Hdac7(56233) ; rat ... Hdac7a(84582)

Description

General description

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1, HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. HDAC7 is predominantly found in the cell nucleus and slightly in cytoplasm. HDAC7 shuttles between these two regions and this is controlled by 14-3-3 protein and calmodulin-dependent kinase I (CaMKI)

Immunogen

synthetic peptide corresponding to amino acids 137-155 of mouse HDAC7 with N-terminal added lysine, conjugated to KLH.

Application

Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• antibody microarray

Biochem/physiol Actions

HDAC7 belongs to class II of HDAC family and combines with MEF2 group of transcription factors, which is facilitated by theN-terminal domain of HDAC7. This binding blocks the transcriptional activity and prevents myogenesis. They are involved in development and differentiation processes in various tissues such as skeletal, cardiac, and smooth muscle, the bone, the immune system, the vascular system, and the brain. These signal-dependent co-repressors phosporylate at the regulatory N-terminal domain resulting in nuclear export.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable