GERPN2235
ECL™ Select Western Blotting Detection Reagent
Cytiva RPN2235
Synonym(s):
chemiluminescent detection reagent, Western blotting detection reagent
About This Item
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packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2235
Related Categories
General description
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
Application
Features and Benefits
- Improved sensitivity: Produces a very high signal intensity
- Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
- Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
- Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes
Components
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
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Protocols
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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