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Key Documents

G100120

Sigma-Aldrich

Sephadex® G-100

Medium

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

Quality Level

form

slurry

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 15-20 mL gel

bead size

40-120 μm

separation technique

size exclusion (SEC)

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General description

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000

Application

Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

Other Notes

G100120-100G′s updated product number is GE17-0060-01

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R H Coleman et al.
Current microbiology, 32(3), 147-150 (1996-03-01)
Pseudomonas syringae isolate BR2R produces tabtoxin, a beta-lactam-containing antibiotic, and the causative agent of wildfire disease of green bean (Phaseolus vulgaris). beta-Lactamase production has been suggested as the mechanism that protects P. syringae from tabtoxin. We sought to determine whether
Production, purification and characterization of an a-amylase produced by <I>Saccharomycopsis fibuligera</I>
Gogoi, B.K., et al.
Journal of Applied Microbiology, 63(5), 373-379 (1987)
Fat metabolism in higher plants. XLIV. Fatty acid synthesis by a soluble fatty acid synthetase from Sclanum tuberosum.
K P Huang et al.
Archives of biochemistry and biophysics, 143(2), 412-427 (1971-04-01)
Ahmed K A El-Sayed et al.
International journal of biological macromolecules, 132, 1274-1281 (2019-04-07)
AmyLa α-amylase gene from Laceyella sp. DS3 was heterologously expressed in E. coli BL21. E. coli BL21 maximally expressed AmyLa after 4 h of adding 0.02 mM IPTG at 37 °C. The recombinant AmyLa α-amylase was purified 2.19-fold through gel filtration and ion
Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles
Pittet MJ, et al.
Nature Protocols, 1(1), 73-73 (2006)

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