Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
F2637
Ficoll® 400
BioXtra, for molecular biology, lyophilized powder
Synonym(s):
Polysucrose 400, Ficoll 400
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5 G
R 1 685,60
25 G
R 5 023,48
100 G
R 12 860,54
R 1 685,60
List PriceR 1 720,00Please contact Customer Service for Availability
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5 G
R 1 685,60
25 G
R 5 023,48
100 G
R 12 860,54
About This Item
R 1 685,60
List PriceR 1 720,00Please contact Customer Service for Availability
Recommended Products
biological source
non-animal source
Quality Level
grade
Molecular Biology
for molecular biology
product line
BioXtra
form
lyophilized powder
foreign activity
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.
room temp
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General description
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Application
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane[1]
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.[2]
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).[3]
Used for cell separation and organelle isolation.
Features and Benefits
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocols
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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Is Product F2637, Ficoll, the same as Ficoll 400?
1 answer-
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What is the difference between Ficoll 400 and Ficoll PM 400?
1 answer-
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 answer-
Please check the product information sheet for F4375 which has a detailed protocol.
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 answer-
When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 answer-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
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