biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-LFA-1, sheep
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
The antibody recognises the sheep LFA-1 molecule expressed by virtually all leukocytes. There is species cross-reactivity with goat.
Immunogen
Sheep lymphocytes
Application
FACS
Culture Medium
IDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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