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product name
Detroit 532, 87032602
biological source
human skin
growth mode
Adherent
karyotype
2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian, skin, Down syndrome
Cell Line Description
Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.
Application
Virus studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, up to 30 passages expected.
Other Notes
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Certificates of Analysis (COA)
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