UM-UC-13
8090508, human bladder, Epithelial
Synonym(s):
Human UM-UC-13, UM-UC-13 Cell Line
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About This Item
UNSPSC Code:
41106514
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Product Name
UM-UC-13, 08090508
biological source
human bladder
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human transitional cell carcinoma lymphatic metastasis
Cell Line Description
UM-UC-13 was produced from a lymphatic metastasis from a transitional cell carcinoma of the bladder. UM-UC-13 produced tumours in female athymic nude mice after 36 days. Susceptibility to adenoviral mediated gene transduction and expression of p53, p16, retinoblastoma protein and coxsackie adenovirus receptor were studied.
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18
Culture Medium
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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