Solutions prepared at 100 mM in water are stable for at least 1 year when stored refrigerated. Note that a prepared solution is available. Please see the link below to review:
https://www.sigmaaldrich.com/product/sigma/19899
C9426
CHAPS hydrate
BioReagent, suitable for electrophoresis, ≥98% (HPLC)
Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
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1 G
R 1 619,94
5 G
R 5 929,00
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1 G
R 1 619,94
5 G
R 5 929,00
About This Item
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NB.22
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description
zwitterionic
Quality Level
product line
BioReagent
Assay
≥98% (HPLC)
form
powder
mol wt
614.88 g/mol (anhydrous basis)
technique(s)
electrophoresis: suitable
protein quantification: suitable
impurities
<0.04% DMF
mp
157 °C (dec.) (lit.)
solubility
water: 50 mg/mL, clear, colorless
suitability
suitable for electrophoresis
storage temp.
room temp
SMILES string
O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI
1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key
SJCUTFKCLFLIFE-JWTJKVBLSA-N
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General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts.[1] It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
CHAPS has been used:
- For membrane permeabilization of cells[2]
- As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue[3][4]
- As a component in denaturing buffer in enzyme characterization[5]
- As a component of 2D- sample buffer in 2D electrophoresis[6]
- For protein solubilization in isoelectric focusing[7]
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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