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C5915

Sigma-Aldrich

Coconut water

suitable for plant cell culture

Synonym(s):

Coconut beverage, Coconut extract, Coconut juice

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About This Item

UNSPSC Code:
10171502
NACRES:
NA.72

sterility

sterile-filtered

form

solution

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

Application

Coconut water is used as a supplement with plant cell culture media such as Murashige & Skoog media.
Use at a concentration of 5-20% (v/v)

Other Notes

Coconut water has been shown to stimulate shoot proliferation in many species of plants. It is prepared from selected coconuts and processed to remove most of the protein. The product is then filter sterilized and frozen prior to shipment. Remaining protein levels in the water may vary from one lot to the next and may result in precipitate when the product is frozen. This precipitation should not effect the growth of the plant tissue. The precipitate can be removed by filtering or by allowing it to settle to the bottom of the bottle and then decanting. Coconut water can be divided into smaller aliquots, corresponding to your standard medium batch size, and refrozen until needed. Coconut water should be used at a concentration of 5-20% (v/v).

Preparation Note

Material is deproteinized

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Porntip Wongkaew et al.
Plant cell reports, 23(6), 426-434 (2004-08-17)
Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l
K P Martin et al.
Indian journal of experimental biology, 43(3), 280-285 (2005-04-09)
Large-scale in vitro propagation protocol for Dendrobium hybrids Sonia 17 and 28, two highly prized commercial cut flower cultivars through shoot multiplication using flower stalk node explants and protocorm-like bodies (PLBs) formation was accomplished. Both hybrids did not exhibit significant
Sungkumlong et al.
Indian journal of experimental biology, 46(4), 243-248 (2008-06-03)
In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media
Suvi T Häkkinen et al.
Plant cell reports, 39(12), 1655-1668 (2020-09-07)
Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and

Protocols

Media Preparation – Plant Tissue Culture Protocol

Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use

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