Recommended Products
form
liquid
Related Categories
Application
BCIP/NBT solution, premixed has been used:
- as a substrate to develop dot-blots
- as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
- to detect specific immune complexes in western blot analysis
Other Notes
0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
Substrates
Substrate for visualizing alkaline phosphatase conjugates in Western blotting.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Slow-and fast-twitch rat hind limb skeletal muscle phenotypes 8 months after spinal cord transection and olfactory ensheathing glia transplantation
Negredo P, et al.
The Journal of Physiology, 586(10), 2593-2610 (2008)
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service