Skip to Content
Merck
All Photos(3)

Documents

A8185

Sigma-Aldrich

Ala-Gln

BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

Alanyl-glutamine, Glutamine-S

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C8H15N3O4
CAS Number:
39537-23-0
Molecular Weight:
217.22
MDL number:
MFCD00133046
UNSPSC Code:
12352209
PubChem Substance ID:
24891211
NACRES:
NA.75

product line

BioReagent

Quality Level

300

Assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

color

white to off-white

storage temp.

room temp

SMILES string

NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O

InChI

1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1

InChI key

HJCMDXDYPOUFDY-WHFBIAKZSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Ala-Gln has been used:
  • as a supplement in NeuralQ™ medium
  • as a component in growth medium and incubation medium (IM) for retinal cell lines
  • for experimental treatment of retinal cell lines respectively

Biochem/physiol Actions

Alanyl-glutamine (Ala-Gln) is a dipeptide that is used as a substitute for glutamine in mammalian cell culture media, because of the instability of glutamine in solution and the formation of ammonia as a breakdown product of glutamine in solution, which can have deleterious effects on cells in culture. Ala-Glu is stable to heat sterilization and is less ammonia genic than glutamine, which contributes to its advantages as a media component. Ala-Gln has been utilized to investigate polymorphonuclear leucocyte and myeloperoxidase activity in vitro.

Analysis Note

This product is cell culture tested (0.5 mg/ml) and insect cell culture tested (0.2 mg/ml). It is appropriate for use in cell culture and insect cell culture applications at 2-10 mM.

Legal Information

NeuralQ is a trademark of GlobalStem

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vinicius Fernandes Cruzat et al.
Nutrition (Burbank, Los Angeles County, Calif.), 25(4), 428-435 (2008-12-06)
We investigated the effect of supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine, both in the free form, on the plasma and tissue concentrations of glutamine, glutamate, and glutathione (GSH) in rats subjected to long-duration
Vinicius Fernandes Cruzat et al.
Cell biochemistry and function, 28(1), 24-30 (2009-11-04)
In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted
The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium
Christie A, et al.
Biotechnology and Bioengineering, 64(3), 298-309 (1999)
A high-throughput assay for mRNA silencing in primary cortical neurons in vitro with oligonucleotide therapeutics
Alterman JF, et al.
Bio-protocol, 7(16) (2017)
T Hassell et al.
Applied biochemistry and biotechnology, 30(1), 29-41 (1991-07-01)
Eight independent cell lines accumulated ammonia in culture to concentrations between 1.3 and 2.9 mM. The growth inhibition of such concentrations of ammonium chloride when added to culture medium was variable. The cell lines tested could be divided into 3
View All Related Papers

Articles

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service