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A3293

Sigma-Aldrich

Anti-Human Albumin antibody produced in rabbit

whole antiserum

Synonym(s):

Albumin Antibody, Albumin Antibody - Anti-Human Albumin antibody produced in rabbit, Anti Human Albumin Antibody

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 66.437-66.6 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

indirect ELISA: 1:60,000
quantitative precipitin assay: 2.4-3.6

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALB(213)

General description

Albumin′s primary structure is composed of a single chain of amino acids and the secondary structure is composed of α-helix of six turns and 17 disulfide bridges. The tertiary structure is 3D and is composed of three domains I II and III. Each of these domains are made of sub-domains IAB, IC, IIAB, IIC, IIIAB, IIIC, respectively.

Specificity

Anti-human albumin antibodies are specific for human albumin.

Immunogen

Human albumin

Application

Anti-Human Albumin antibody has been used in enzyme linked immunosorbent assay (ELISA) and in immobilization of antibodies for human serum albumin (HSA).
Anti-human albumin antibody may be used in quantitative precipitin assay, high-throughput protein microarray analysis and immunocytochemistry .
Rat hepatocytes were grown on collagen-coated coverslips, fixed in formalin and permeablized with methanol prior to incubation with rabbit anti-human albumin antibody.

Biochem/physiol Actions

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions among several others . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes .
Albumin plays a major role in transportation and deposition of many exogenous and endogenous substances in blood.

Physical form

Supplied as a liquid containing 15mM sodium azide as preservative.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Sreedevi Karthi et al.
Experimental and therapeutic medicine, 23(1), 82-82 (2021-12-23)
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Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling
Gelamo E L, et al.
Biochimica et Biophysica Acta, Protein Structure and Molecular Enzymology, 1594(1), 84-99 (2002)

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