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3017

Sigma-Aldrich

Transcreener® EPIGEN Methyltransferase Assay

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R 25 555,46
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R 153 095,60

R 25 555,46

List PriceR 26 077,00
Please contact Customer Service for Availability
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1 EACH
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About This Item

UNSPSC Code:
41122100
NACRES:
NA.84

R 25 555,46

List PriceR 26 077,00
Please contact Customer Service for Availability

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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Quantity

3017-1K = 1,000 assay, 384-well
3017-10K = 10,000 assay, 384-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

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