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00062107

MDCK-II Cell Line canine

Synonym(s):

MDCK Type II Cells, MDCK-2 Cells, MDCKII-WT Cells

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81
Pricing and availability is not currently available. Contact Technical Service

biological source

canine kidney

Quality Level

100

description

Canine Cocker Spaniel Kidney

form

liquid

growth mode

Adherent

karyotype

Dog

morphology

Polarised epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Canine Cocker Spaniel Kidney

Cell Line Description

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.

Culture Medium

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C,

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Manuel S Brugger et al.
Biosensors & bioelectronics, 173, 112807-112807 (2020-11-23)
A Love-wave based biosensor is introduced for analyzing a standardized wound healing assay by observing cell growth and quantifying cell detachment processes. Utilizing the piezoelectric material LiTaO3 36° XY-cut with a thin SiO2-cover layer, shear horizontal surface acoustic waves (SAW)
Olga Danov et al.
Frontiers in medicine, 7, 571003-571003 (2020-11-27)
Smokers with apparently "healthy" lungs suffer from more severe and frequent viral respiratory infections, but the mechanisms underlying this observation are still unclear. Epithelial cells and dendritic cells (DC) form the first line of defense against inhaled noxes such as
D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was

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Questions

  1. Dear ECACC, We are currently developing critical assays using this MDCK-II cell line (ECACC 00062107) at Genentech. I hope to ask your help to get any gene or protein expression database of this cell line. Any published data available? Thank you!

    1 answer

    1. This cell line is a product of ECACC. Please see the information below to review the ECACC product and contact information, original citation, and depositor.

      ECACC MDCK II product page:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=00062107&collection=ecacc_gc

      ECACC contact form:
      https://www.culturecollections.org.uk/contactus/index.aspx

      Link to original cited reference:
      https://pubmed.ncbi.nlm.nih.gov/3519211/

      Cell Line Depositor:
      Professor Gerrit van Meer, Dept. of Membrane Enzymology, Centre for Biomembranes and Lipid Enzymology, H.R Kruytgebouw N607, Padualaan 8, 3584 CH Utrecht, The Netherlands.

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