bioburden testing environmental food and beverages water monitoring microbiology
suitability
selective and differential for Enterobacter spp. selective and differential for Escherichia coli selective and differential for Klebsiella spp. selective and differential for Proteus spp. selective and differential for Salmonella spp. selective and differential for Shigella spp. selective and differential for coliforms
Application
A selective medium for the differentiation of lactose fermenting and lactose non-fermenting intestinal organisms.
Preparation Note
Suspend 41 g in 1 litre of distilled water. Add 4 ml of 10% w/v alcoholic solution (96% ethyl alcohol) of basic fuchsin (Cat. No. 47860). Bring to boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well before pouring.
Other Notes
The assessment of ammonia-induced cell envelope injury in E. coli and Enterobacter aerogenes.[1]
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Canadian journal of microbiology, 36(8), 525-529 (1990-08-01)
Ammonia-induced cell envelope injury was examined in pure cultures of Escherichia coli and Enterobacter aerogenes. Cell injury, as determined by the ratio of colony-forming units on m-T7 agar to colony-forming units on m-Endo agar, increased with exposure to increasing concentrations
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