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66725

Sigma-Aldrich

Methylene Blue solution alkaline according to Loeffler

according to Loeffler

Synonym(s):

Methylene Blue solution, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12352200
PubChem Substance ID:
329760312
NACRES:
NA.25

Quality Level

100

shelf life

limited shelf life, expiry date on the label

composition

dye (C.I.No. 52015), 0.3 % (w/v) (in ethanol:0.1% aqueous KOH 3:10)

refractive index

n20/D 1.347

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

Application

Loeffler′s alkaline methylene blue stain solution has been used for the staining of microalgae cells.

Other Notes

Prepared according to Clark

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

84.2 °F - closed cup

Flash Point(C)

29 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCC3086
BCBV6776
BCBQ7485V
BCBM4441V
BCBH8983V

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G. Clark
Staining Procedures, 380-380 (1981)
Associated effects of storage and mechanical pre-treatments of microalgae biomass on biomethane yields in anaerobic digestion.
Gruber-Brunhumer MR et al.
Biomass and Bioenergy, 93, 259-268 (2016)
Jonel Steffen et al.
The British journal of ophthalmology, 98(1), 13-15 (2013-10-26)
To evaluate the diagnostic accuracy of methylene blue used as a non-invasive in vivo stain to detect ocular surface squamous neoplasia (OSSN). A test validation study was performed according to Standards for the reporting of diagnostic accuracy studies (STARD) guidelines
Nicolaas Lucius Gerlach et al.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 72(4), 666-671 (2014-02-01)
To establish the accuracy of cone-beam computed tomographic (CBCT) views in determining the position and diameter of the mandibular canal. Two freshly frozen cadaver heads, 1 dentate and 1 edentate, were used to acquire CBCT scans. Measurements on cross-sectional CBCT
Q R J G Tummers et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 40(7), 850-858 (2014-05-28)
Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of
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