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410217

Sigma-Aldrich

2′,7′-Dichlorofluorescein

ACS reagent

Synonym(s):

Dichlorofluorescein; Fluorescein 27

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About This Item

Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
76-54-0
Molecular Weight:
401.20
Beilstein:
58009
EC Number:
200-968-6
MDL number:
MFCD00005047
UNSPSC Code:
12171500
PubChem Substance ID:
24865700
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

powder

color

orange to red-brown, powder

mp

280 °C (dec.) (lit.)

solubility

alcohol: passes test

density

0.790 g/cm3

λmax

509 nm

suitability

passes test for adsorption indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Application

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL8813
BCCK3080
BCCH5169
BCCF7954
BCBG1058V

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Archives of biochemistry and biophysics, 527(1), 55-64 (2012-08-23)
Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells
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Toxicology in vitro : an international journal published in association with BIBRA, 24(6), 1512-1520 (2010-07-20)
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Journal of cellular biochemistry, 113(1), 174-183 (2011-09-02)
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