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Supelco

Bispyrazolone

for TLC derivatization, for the det.of cyanide, ≥98.0%

Synonym(s):

3,3′-Dimethyl-1,1′-diphenyl-(4,4′-bi-2-pyrazoline)-5,5′-dione

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About This Item

Empirical Formula (Hill Notation):
C20H18N4O2
CAS Number:
Molecular Weight:
346.38
Beilstein:
572787
EC Number:
MDL number:
UNSPSC Code:
41116105
PubChem Substance ID:
NACRES:
NA.22

grade

for TLC derivatization

Quality Level

Assay

≥98.0% (TLC)
≥98.0%

form

powder

quality

for the det.of cyanide

technique(s)

thin layer chromatography (TLC): suitable

solubility

formic acid: 50 mg/mL, clear, yellow

SMILES string

CC1=NN(c2ccccc2)C(=O)C1C3C(C)=NN(c4ccccc4)C3=O

InChI

1S/C20H18N4O2/c1-13-17(19(25)23(21-13)15-9-5-3-6-10-15)18-14(2)22-24(20(18)26)16-11-7-4-8-12-16/h3-12,17-18H,1-2H3

InChI key

FORCWSNQDMPPOC-UHFFFAOYSA-N

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Application

Bispyrazolone was used in an experimental procedure, done in order to remove linamarin in starch from cassava roots, by using plant cell wall-degrading enzymes, xylanase and cellulase. It was also used for spectrophotometric determination and quantization of urea in urine samples.

Other Notes

Determination of ammonia; Assay of cyanogenic compounds

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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L. Prochazkova
Analytical Chemistry, 36, 865-865 (1964)
Spectrophotometric Determination of Urea in Urine Samples by using Bispyrazolone Method.
Sharma V
Proceedings of the Indian Academy of Sciences - Section A, 79 (1) (2013)
An enzymatic assay for the total cyanide content of cassava (Manihot esculenta Crantz).
R D Cooke
Journal of the science of food and agriculture, 29(4), 345-352 (1978-04-01)
An efficient treatment for detoxification process of cassava starch by plant cell wall-degrading enzymes.
Journal of Bioscience and Bioengineering, 109 (1), 9-14 (2010)
R.D. Cooke et al.
Journal of the Science of Food and Agriculture, 33, 269-269 (1982)

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