0029-BP

GlcNAcβ-C3-PAA-biot

• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• carrier: PAA carrier
• conjugate: biotin conjugate
• product line: BP-Series
• Assay: 95% (H-NMR)
• form: lyophilized powder
• mol wt: ~20,000 Da
• manufacturer/tradename: GlycoNZ
• extent of labeling: 20 mol% Biotin ligand density
• technique(s): flow cytometry: suitable; glycol-bead construction: suitable; glycol-surface construction: suitable
• probe type: monosaccharide
• probe presentation: polymeric
• application(s): histochemistry
• storage temp.: −20°C

Description

General description

BP-Series: Biotinylated Polymeric Probes
This series of glycoconjugate probes includes HOCH₂CH₂NH₃⁺-salts for acidic sugars. The affinity of these probes is 10² to 10⁵ times higher than that of the corresponding free sugars. The spacer-arm is normally –(CH₂)₃–, while the spacer arm for biotin is –(CH₂)₆–. The flexible polymer chain behaves as an additional spacer.

Used as a high molecular weight carrier, poly[N-(2-hydroxyethyl)acrylamide], abbreviated as PAA, has low non-specific sorption, and is stable to chemical and proteolytic action.

Application

Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Preparation Note

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and ¹H-NMR)

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable