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11-0398

Sigma-Aldrich

Fluorescamine

≥98.0%

Synonym(s):

4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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About This Item

Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
38183-12-9
Molecular Weight:
278.26
Beilstein:
921143
EC Number:
253-814-5
MDL number:
MFCD00005928
UNSPSC Code:
12191502
PubChem Substance ID:
329749297

Assay

≥98.0%

availability

available only in Japan

mp

153-157 °C (lit.)

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

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Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Suitability

for HPLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Min Jia et al.
Analytical chemistry, 81(19), 8033-8040 (2009-10-01)
Fluorescamine derivatized 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (I) is shown to undergo an irreversible reaction with peroxyl radicals and other radical oxidants to generate a more highly fluorescent diamagnetic product (II) and thus can be used as a highly sensitive and versatile probe to
Amit K Jha et al.
Biomaterials, 32(10), 2466-2478 (2011-01-11)
We have created hyaluronic acid (HA)-based, cell-adhesive hydrogels that direct the initial attachment and the subsequent differentiation of human mesenchymal stem cells (MSCs) into pre-osteoblasts without osteogenic supplements. HA-based hydrogel particles (HGPs) with an average diameter of 5-6 μm containing
Manjeet Deshmukh et al.
Biomaterials, 31(26), 6675-6684 (2010-06-22)
Two vinyl sulfone functionalized crosslinkers were developed for the purpose of preparing degradable poly(ethylene glycol) (PEG) hydrogels (EMXL and GABA-EMXL hydrogels). A self-elimination degradation mechanism in which an N-terminal residue of a glutamine is converted to pyroglutamic acid with subsequent
Wen-Hsien Tsai et al.
Journal of chromatography. A, 1217(49), 7812-7815 (2010-11-04)
A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine
Zeynep Aydoğmuş
Journal of fluorescence, 19(4), 673-679 (2009-02-03)
A new, simple and sensitive spectrofluorimetric method has been developed for the determination of oseltamivir phosphate (OSP) in capsules. The method is based on the reaction between oseltamivir and fluorescamine in borate buffer solution of pH 8.50 to give highly
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