TAQN-RO

Taq DNA Polymerase, dNTPack

suitable for PCR, optimum pH ~9.0 (20 °C), dNTPs included

• Synonym(s): dna amplification, pcr, polymerase, primer extension
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• biological source: bacterial (Thermus aquaticus BM)
• Quality Level: 100
• recombinant: expressed in E. coli
• description: 5 U/βl; Number of Reactions:; If 1.25 U are used per 50 μL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage.
• form: liquid
• usage: sufficient for 2,000 reactions (04728904001); sufficient for 4,000 reactions (04728858001); sufficient for 400 reactions (04728874001); sufficient for 80 reactions (04728866001); sufficient for 800 reactions (04728882001)
• mol wt: 95 kDa
• feature: dNTPs included; hotstart: no
• packaging: pkg of 1,000 U (04728882001 [4 x 250 U]); pkg of 2,500 U (04728904001 [10 x 250 U]); pkg of 5,000 U (04728858001 [20 x 250 U]); pkg of 100 U (04728866001); pkg of 500 U (04728874001 [2 x 250 U])
• manufacturer/tradename: Roche
• concentration: 0.025 units/reaction
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• optimum pH: ~9.0 (20 °C)
• solubility: water: soluble
• suitability: suitable for PCR and automated sequencing reactions
• UniProt accession no.: (P19821 · DPO1_THEAQ)
• application(s): genomic analysis; life science and biopharma
• foreign activity: endonucleases, none detected; exonuclease, none detected; nicking activitities, none detected
• storage temp.: −20°C

Description

General description

Contents
Taq DNA Polymerase, 5 U/μl
PCR Buffer, 10x concentrated, with MgCl₂
PCR Nucleotide Mix

For maximum convenience, select the ready-to-use 2x concentrated PCR Master.

Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.

Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.

Application

Achieve the most consistent results in simple, routine PCR by using Roche Applied Science Taq DNA Polymerase, which is held to our rigorous purity and quality standards. The Taq DNA Polymerase, dNTPack, combines our standard preparation (5 U/μl) of recombinant Taq DNA Polymerase with our convenient, ready-to-use PCR Nucleotide Mix. In all other respects, this preparation is identical to our standard preparation and can be used for:

• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Features and Benefits

• Reliable reproducible results: High lot-to-lot consistency.
• No need to test each lot: Taq DNA Polymerase is rigorously tested.
• Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

Packaging

1 kit containing 3 components

Quality

Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Storage and Stability

Containers which are opened must be carefully resealed and
kept upright to prevent leakage

Other Notes

For life science research only. Not for use in diagnostic procedures.

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.

Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

• Taq DNA Polymerase 5 U/μl
• PCR Buffer with MgCl₂ 10x concentrated
• PCR Nucleotide Mix

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash