form
solution
packaging
pkg of 200 U (10 U/μl)
manufacturer/tradename
Roche
parameter
25 °C optimum reaction temp.
shipped in
dry ice
storage temp.
−20°C
Specificity
Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.
Application
Swa I is very useful for digesting GC-rich bacterial genomes.
DNA Profile
Number of cleavage sites on different DNAs
- λ: 0
- φX174: 0
- Ad2: 1
- M13mp7: 1
- pBR322: 0
- pBR328: 0
- SV40: 1
Unit Definition
One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.
Analysis Note
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
The buffer in bold is recommended for optimal activity
- A: 0-10%
- B: 10-25%
- H: 100%
- L: 0-10%
- M: 0-10%
Activity in PCR buffer: Not tested
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Enzyme Solution
- SuRE/Cut Buffer H 10x concentrated
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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