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Roche

BM Condimed H1

Hybridoma Cloning Supplement

Synonym(s):

hybridoma cloning supplement

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About This Item

UNSPSC Code:
12352207

Quality Level

100

sterility

0.2 μm filtered

form

solution

packaging

pkg of 100 mL (10x)

manufacturer/tradename

Roche

technique(s)

cell culture | hybridoma: suitable

solubility

water: miscible

storage temp.

−20°C

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General description

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Cell Line Origin

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Application

BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

Features and Benefits

Contents
Solution in RPMI 1640, filtered through 0.2μm pore size membrane

Physical form

Solution in RPMI 1640, filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.

Analysis Note

Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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Mark F Maurer et al.
mAbs, 4(1), 69-83 (2012-02-14)
Interleukin-21 (IL-21) is a type I four-helical bundle cytokine that exerts a variety of significant effects on many hematopoietic cells, including T and B lymphocytes and natural killer cells. IL-21 is produced predominantly by CD4+ T cells and natural killer
Christine J Rossant et al.
mAbs, 6(6), 1425-1438 (2014-12-09)
Generation of functional antibodies against integral membrane proteins such as the G-protein coupled receptor CXCR2 is technically challenging for several reasons, including limited epitope accessibility, the requirement for a lipid environment to maintain structure and their existence in dynamic conformational
Atsuko Y Higashi et al.
Scientific reports, 11(1), 21642-21642 (2021-11-06)
The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (TJs). Although FS

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