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10223581001

Roche

Poly(dA)

Poly-deoxy-adenylic acid

Synonym(s):

PCR

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About This Item

UNSPSC Code:
41105600
Pricing and availability is not currently available.

form

lyophilized

mol wt

330 Da

packaging

pkg of 5 U

manufacturer/tradename

Roche

absorbance ratio

A290/260 nm 0.03-0.12
A280/260 nm 0.28-0.39
A250/260 nm 0.84-0.94

storage temp.

−20°C (−15°C to −25°C)

General description

Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units.[1] Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.[2]

Application

Poly(dA) is suitable for use in:
  • DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies[3]
  • as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis[4]
  • in Pyrococcus abyssi DNA polymerase assay[5]

Sequence

Chain Length Mean chain length lot 20-17: 250-500 bases.

Unit Definition

1 A260 unit corresponds to 40 μg ssDNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
11534322
11534321
14405323
14405320
14405322

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V Iyer et al.
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
A specific photoreaction in polydeoxyadenylic acid
Porschke D
Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)
Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G 40 P, interacts with forked DNA
Ayora S, et al.
Nucleic Acids Research, 30(11), 2280-2289 (2002)
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
Maga G, et al.
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
Henneke G, et al.
Journal of molecular biology, 350(1), 53-64 (2005)
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