Please see the links below to review the 'Immobilon® ECL Ultra Western HRP Substrate User Guide' and the 'Immobilon® Forte Western HRP Substrate' datasheet. Both of these documents show comparative data regarding the sensitivity of this substrate. User Guide https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/151/492/wbuls0x00-ver1-0916.pdf Datasheet https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/174/618/20331986-rev0518.pdf
WBULS0500
Immobilon ECL Ultra Western HRP Substrate
Western blotting, HRP
Synonym(s):
chemiluminescent detection substrate, detection substrate
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500 ML
R 13 024,20
R 13 024,20
List PriceR 13 290,00Please contact Customer Service for Availability
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500 ML
R 13 024,20
About This Item
UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NA.32
R 13 024,20
List PriceR 13 290,00Please contact Customer Service for Availability
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General description
Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.
Application
"Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.
Sufficient for 5000 cm²"
Sufficient for 5000 cm²"
Western blotting, HRP
Components
Solution A- 250 mL
Solution B- 250 mL
Solution B- 250 mL
Storage and Stability
Store at 2–8 °C
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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