The use of Matrigel is not recommended, as it is not well-defined and may activate adhesion and signaling receptors that promote differentiation.
SF-CMTI-2
ESGRO® Complete Adapted C57/BL6 Mouse ES Cell Line
Mouse
Synonym(s):
BRUCE-4 ES Cells, BRUCE-4 ES cells, CMTI-2 Mouse ES Cells
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2 VIALS
R 24 025,68
R 24 025,68
List PriceR 24 516,00Please contact Customer Service for Availability
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2 VIALS
R 24 025,68
About This Item
UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.52
biological source:
mouse
manufacturer/tradename:
Specialty Media
R 24 025,68
List PriceR 24 516,00Please contact Customer Service for Availability
Request a Bulk Order
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Product Name
ESGRO® Complete Adapted C57/BL6 Mouse ES Cell Line, The Embryonic Stem Cell Lines derived from murine strain C57/BL6J are selected for high targeting efficiency & proven germ line transmission.
biological source
mouse
manufacturer/tradename
Specialty Media
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
General description
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 12. This ES cell line has been pre-adapted to serum-free and feeder-free cell culture conditions and is intended for use with the ESGRO Complete cell culture system.
Cell Line Description
Embryonic Stem Cells
Application
Research Category
Stem Cell Research
Stem Cell Research
Packaging
2.5x106 ea
Storage and Stability
Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.
Legal Information
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Meifang Li et al.
Chinese medicine, 15, 83-83 (2020-08-11)
Huyang Yangkun Formula (HYYKF) was developed based on theory of traditional Chinese medicine as well as clinical experience and used to improve ovarian function of premature ovarian insufficiency (POI) patients. Transplantation of embryonic stem cells (ESCs) has great potential in
Jixue Li et al.
The CRISPR journal, 3(6), 470-486 (2020-11-05)
We describe a protocol for the precise integration of exogenous DNA into user-defined genomic loci in cultured cells. This strategy first introduces a promoter and a lox site to a specific location via a Cas9-induced double-strand break. Second, a gene
Baisakhi Mondal et al.
eLife, 9 (2020-04-17)
The mitotic deacetylase complex (MiDAC) is a recently identified histone deacetylase (HDAC) complex. While other HDAC complexes have been implicated in neurogenesis, the physiological role of MiDAC remains unknown. Here, we show that MiDAC constitutes an important regulator of neural
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Do you have any insights on whether Matrigel coating is not preferable for maintaining ESC pluripotency?
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