SCC112
FLAG-SNAP-hTERT expressing HeLa Cell Line
Human
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
product name
FLAG-SNAP-hTERT expressing HeLa Cell Line, FLAG-SNAP-hTERT expressing HeLa Cell Line is a useful tool for studying telomerase biology and enables purification and visualization of endogenous telomerase reverse transcriptase (TERT).
biological source
human
Quality Level
shipped in
ambient
Related Categories
General description
Telomerase play a critical role in regulating proliferation and senescence in normal somatic cells as well as most cancer cells. In humans, the telomerase reverse transcriptase (hTERT) is a lowly expressed protein with only several hundred molecules per cell. The low expression has made it difficult to detect, purify and study TERT activity from the endogenous loci.
To facilitate reliable detection of the endogenous TERT protein, a FLAG-SNAP tag was fused to the N-terminus of the TERT locus in HeLa cells. The FLAG-SNAP tag does not impair the function of the endogenous hTERT protein. Efficient immunopurification (IP) of the active telomerase ribonucleoprotein complexes may be accomplished by using the well-characterized FLAG antibody. The SNAP tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.
Reference:
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.
Reference:
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.
Cell Line Description
Cancer Cells
Application
Research Category
Cell Culture
Cell Culture
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Quality
• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Cells are positive for HPV-18 by PCR.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Cells are positive for HPV-18 by PCR.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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