AB3786
Anti-Prosurfactant Protein C (proSP-C) Antibody
serum, Chemicon®
Synonym(s):
proSP-C
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SFTPC(6440) , SP5(389058)
General description
Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.
Specificity
Expected to cross-react with human.
Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.
Immunogen
GST-tagged recombinant protein corresponding to the N-terminal region of human Prosurfactant Protein C (proSP-C).
Application
- Western Blotting Analysis: A 1:500 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue lysate and GST-fusion Prosurfactant Protein C fragment.
- ELISA Analysis: A 1:50,000 dilution from a representative lot detected GST-fusion Prosurfactant Protein C fragment.
- Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
This rabbit polyclonal Anti-Prosurfactant Protein C (proSP-C), Cat. No. AB3786 is tested for use in Western Blotting, Immunohistochemistry (Paraffin) and ELISA, for the detection of Prosurfactant Protein C (proSP-C).
Quality
Evaluated by Western Blotting in rat lung tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Prosurfactant Protein C (proSP-C) in rat lung tissue lysate.
Target description
20 kDa observed. 21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: 07-647
Physical form
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Mouse lung epithelial cells.
Mouse lung epithelial cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Christian C Hofer et al.
American journal of physiology. Lung cellular and molecular physiology, 308(7), L628-L638 (2015-01-18)
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