AB10002
Anti-mouse BID cleavage (59/60) site specific Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Bid(12122)
General description
BH3 interacting domain death agonist (BID, 22 kDa) is a pro-apoptotic member of the Bcl-2 family. BID interacts with both Bcl-2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase-8 in response to TNF-α or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.
Specificity
Mouse 15 kDa caspase-8 cleavage product of BID. Human (75% conserved) BID has been tested and shown not to cross-react. Rat (100% conserved) and chicken (62% conserved) BID have not been tested.
Immunogen
The antiserum was produced against a chemically synthesized peptide derived from the 15 kDa caspase-8 cleavage product of mouse BID.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
This Anti-mouse BID cleavage (59/60) site specific Antibody is validated for use in WB for the detection of mouse BID cleavage (59/60) site specific.
Physical form
Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier, 0.05% sodium azide.
Format: Purified
Storage and Stability
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Analysis Note
Control
Mouse L929 (3T3-L1) cells treated with TNF-α to induce cleavage; caspase-8 cleaved recombinant mouse BID.
Mouse L929 (3T3-L1) cells treated with TNF-α to induce cleavage; caspase-8 cleaved recombinant mouse BID.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hongyan Sun et al.
BMC genomics, 23(1), 341-341 (2022-05-04)
Receptor interacting serine/threonine kinase 2 (RIP2), ubiquitous in many tissue/cell types, is the key regulator of immune and inflammatory responses for many diseases, including avian pathogenic E. coli (APEC), which causes a wide variety of localized or systemic infections. However
Targeting the BH3-interacting domain death agonist to develop mechanistically unique antidepressants.
Malkesman, O; Austin, DR; Tragon, T; Henter, ID; Reed, JC; Pellecchia, M; Chen, G; Manji, HK
Molecular Psychiatry null
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