208748

Calpain-1 Substrate, Fluorogenic

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

• Synonym(s): Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
• Empirical Formula (Hill Notation): C₇₉H₉₅N₁₃O₁₉S₂
• Molecular Weight: 1594.81
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: red
• solubility: DMSO: 1 mg/mL
• fluorescence: λ_ex ~490 nm; λ_em ~518 nm
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable