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Sigma-Aldrich

Anti-phospho-Adducin (Ser662) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, canine, human, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... ADD3(120)

Specificity

phosphorylated serine 662 of γ-Adducin, phosphorylated serine 724 of α-Adducin, phosphorylated serine 713 of β-Adducin

Immunogen

peptide (C-KKFRTPpSFLKKNK) corresponding to amino acids 656-668 of human gamma-Adducin

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-phospho-Adducin (Ser662) Antibody is validated for use in WB for the detection of phospho-Adducin (Ser662).

Quality

routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells or on canine MDCK cells

Target description

80kDa, 120kDa, 110kDa

Physical form

0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.
ImmunoAffinity Purified

Storage and Stability

1 year at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrew M Hau et al.
The American journal of pathology, 189(9), 1846-1862 (2019-06-15)
The mammalian target of rapamycin (mTOR) and associated phosphatidylinositol 3-kinase/AKT/mTOR signaling pathway is commonly up-regulated in cancer, including bladder cancer. mTOR complex 2 (mTORC2) is a major regulator of bladder cancer cell migration and invasion, but the mechanisms by which
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Sequeira, et al.
iScience, 26, 106240-106240 (2023)
Transformation-sensitive changes in expression, localization, and phosphorylation of adducins in renal proximal tubule epithelial cells.
Fowler, L, et al.
Cell Growth & Differentiation : the Molecular Biology Journal of the American Association For Cancer Research, 9, 177-184 (1998)
L Dong et al.
The Journal of biological chemistry, 270(43), 25534-25540 (1995-10-27)
We recently cloned a partial cDNA (35H) for a protein kinase C (PKC) binding protein from a rat kidney cDNA library and demonstrated that it is a PKC substrate in vitro (Chapline, C., Ramsay, K., Klauck, T., and Jaken, S.

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