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HPLC Analysis of 3,4-Dihydroxyphenylalanine (DOPA) Enantiomers on Astec® CHIROBIOTIC® T

HPLC Analysis of 3,4-Dihydroxyphenylalanine (DOPA) Enantiomers on Astec® CHIROBIOTIC® T application for HPLC

Materials

analytical column

Product No.
Description
Pricing

12024AST

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

D9503

3,4-Dihydroxy-DL-phenylalanine

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

0.9 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

5 μL

sample

2 mg/mL in methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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