Urokinase from human kidney cells

Name: Urokinase from human kidney cells
Brand: SIGMA

lyophilized powder

Synonym(s):

u-Plasminogen activator

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About This Item

CAS Number:

9039-53-6

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-917-9

NACRES:

NA.54

MDL number:

MFCD00132566

EC Number:

3.4.21.73 (BRENDA, IUBMB)

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Properties

Quality Segment

200

form

lyophilized powder

mol wt

heavy chain 30,400 Da, light chain 2000 Da

storage temp.

2-8°C

Description

General description

Supplied as a lyophilized powder containing human albumin, mannitol and NaCl.

The principle active ingredient is the low molecular weight form of urokinase, and consists of an A chain of 2,000 daltons linked by a sulfhydyl bond to a B chain of 30,400 daltons.

Application

Urokinase from human kidney cells has been used as a component of digestion mix for three-dimensional fibrin culture. It has also been used in plasminogen activation assay.

Biochem/physiol Actions

Urokinase is a protease that cleaves plasminogen to form plasmin. It binds to cell surface receptors (uPAR) from which it regulates cell adhesion and migration during tissue remodeling and which also activate intracellular signaling pathways. Urokinase is composed of three domains: the amino terminal domain which is homologous to epidermal growth factor is attached through a central kringle domain to the protease domain in the carboxyl terminus.

Urokinase was shown to augment proliferation of renal tumor cells. [1]

Other Notes

Note: Higher activities are obtained when human plasminogen is used as substrate.

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This Item	62970	SRP3137	T7513
Sigma-Aldrich U4010 Urokinase from human kidney cells	Sigma-Aldrich 62970 Lysozyme from chicken egg white	Sigma-Aldrich SRP3137 PAI-2 human	Sigma-Aldrich T7513 Thrombin from bovine plasma
form lyophilized powder	form powder	form lyophilized	form lyophilized powder
mol wt heavy chain 30,400 Da, light chain 2000 Da	mol wt single-chain 14.3 kDa, M_r ~14600	mol wt 60 kDa	mol wt heavy chain ~33 kDa, light chain ~5 kDa
Quality Level 200	Quality Level 100	Quality Level -	Quality Level 200
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C