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RAB0477

Sigma-Aldrich

Mouse Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):

Cachectin, TNF-alpha, Tumor necrosis factor alpha

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Tnf(21926)

General description

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.

Immunogen

Recombinant Mouse TNF-α

Application

Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.

Biochem/physiol Actions

Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mangiferin inhibits apoptosis and oxidative stress via BMP2/Smad-1 signaling in dexamethasone-induced MC3T3-E1 cells
Ding LZ, et al.
International Journal of Molecular Medicine, 41(5), 2517-2526 (2018)
Tumor necrosis factor alpha: a link between neuroinflammation and excitotoxicity
Olmos G and Llado J
Mediators of Inflammation (2014)
Pharmacokinetic and pharmacodynamic characterisation of an anti-mouse TNF receptor 1 domain antibody formatted for in vivo half-life extension
Goodall LJ, et al.
PLoS ONE, 10(9), e0137065-e0137065 (2015)
Curculigoside exerts significant anti-arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF-kappaB signaling pathway
Tan S, et al.
Molecular Medicine Reports, 19(3), 2057-2064 (2019)
Ying Wang et al.
Journal of cellular and molecular medicine, 24(11), 6107-6119 (2020-04-24)
Non-alcoholic fatty liver disease (NAFLD) serves as the most common subtype of liver diseases and cause of liver dysfunction, which is closely related to obesity and insulin resistance. In our study, we sought to investigate effect of transcription factor grainyhead-like
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