PIR02

Inhibitor Resistant Genotyping PCR ReadyMix^™, ROX^™

with ROX for ABI instruments

• Synonym(s): Inhibitor Resistant Genotyping PCR ReadyMix^™
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• feature: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart
• storage condition: protect from light
• technique(s): qPCR: suitable
• color: light blue
• input: crude DNA
• compatibility: ABI 5700; ABI 7000; ABI 7300; ABI 7700; ABI 7900 HT ; ABI 7900 HT Fast; ABI 7900; ABI StepOnePlus
• detection method: probe-based
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use PCR master mix used for amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.

250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable