P9827
Polycytidylic acid–Agarose
lyophilized powder
Synonym(s):
Poly(C)–Agarose potassium salt
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form
lyophilized powder
extent of labeling
0.25-1.0 mg per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
swelling
1 g swells to ~5 mL
storage temp.
−20°C
Application
Polycytidylic acid-agarose is used in protein chromatography, affinity chromatography and specialy resins. Polycytidylic acid has been used to study the preferential induction of apoptosis in the rainbow trout macrophage cell line. Polycytidylic acid has also been used to develop two analytical methods for human secretory-type ribonuclease.
Physical form
Lyophilized powder stabilized with lactose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Preparation of sepharose-bound poly (rI:rC).
A F Wagner et al.
Biochemical and biophysical research communications, 45(1), 184-189 (1971-10-01)
D Nadano et al.
Analytical biochemistry, 212(1), 111-116 (1993-07-01)
Two analytical methods for human secretory-type ribonuclease, which are based on polycytidylic acid/ethidium bromide fluorescence, have been developed. The first is a method for measurement of secretory-type ribonuclease activity utilizing the radial diffusion of ribonuclease in a thin agarose gel
C-P Benny Yiu et al.
Biochemical and biophysical research communications, 343(1), 311-318 (2006-03-18)
Dribble (DBE) is an essential protein in Drosophila that belongs to the evolutionarily conserved Krr1p protein family. Proteins in this family are localised in the cell nucleolus and are important for the processing of ribosomal RNAs. However, little is known
S J Dewitte-Orr et al.
Fish & shellfish immunology, 18(4), 279-295 (2004-11-25)
The rainbow trout macrophage cell line RTS11 was found to be considerably more sensitive than rainbow trout fibroblast (RTG-2) and Chinook salmon epithelial (CHSE-214) cell lines to killing by macromolecular synthesis inhibitors, actinomycin D (AMD) and cycloheximide (CHX), a synthetic
Cristiana Valle et al.
Parasitology research, 96(4), 199-205 (2005-04-15)
We have cloned, sequenced and expressed a gene of Haemonchus contortus that encodes a protein (termed HcCYP) consisting of a cyclophilin domain and an RNA recognition motif (RRM). An antiserum raised against the recombinant protein showed that HcCYP was present
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